Open AccessNoncontiguous finished genome sequence and description of Raoultibacter massiliensis gen. nov., sp. nov. and Raoultibacter timonensis sp. nov, two new bacterial species isolated from the human gut
Author(s) -
Traore Sory Ibrahima,
Bilen Melhem,
Beye Mamadou,
Diop Awa,
Mbogning Fonkou Maxime Descartes,
Tall Mamadou Lamine,
Michelle Caroline,
Yasir Muhammad,
Ibraheem Azhar Esam,
Bibi Fehmida,
Bittar Fadi,
JimanFatani Asif Ahmad,
Daoud Ziad,
Cadoret Fréderic,
Fournier PierreEdouard,
Edouard Sophie
Publication year - 2019
Publication title -
microbiologyopen
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.881
H-Index - 36
ISSN - 2045-8827
DOI - 10.1002/mbo3.758
Subject(s) - 16s ribosomal rna , biology , strain (injury) , phylogenetic tree , microbiology and biotechnology , whole genome sequencing , genus , feces , genome , agar plate , human feces , genomic dna , bacteria , zoology , gene , genetics , anatomy
As part of the culturomics project aiming at describing the human microbiota, we report in this study the description of the new bacterial genus Raoultibacter gen. nov. that includes two new species, that is , R. massiliensis sp. nov. and R. timonensis sp. nov. The R. massiliensis type strain Marseille‐P2849 T was isolated from the fecal specimen of a healthy 19‐year‐old Saudi Bedouin, while R. timonensis type strain Marseille‐P3277 T was isolated from the feces of an 11‐year‐old pygmy female living in Congo. Strain Marseille‐P2849 T exhibited 91.4% 16S rRNA sequence similarity with Gordonibacter urolithinfaciens , its phylogenetic closest neighbor with standing in nomenclature. As well, strain Marseille‐P3277 T exhibited 97.96% 16S rRNA similarity with strain Marseille‐P2849 T . Both strains were Gram‐positive, motile, nonspore‐forming rod and form transparent microcolonies on blood agar in both anaerobic and microaerophilic atmospheres. The genome sizes of strain Marseille‐P2849 T and strain Marseille‐P3277 T were 3,657,161 bp and 4,000,215 bp, respectively. Using a taxono‐genomic approach combining the phenotypic, biochemical, and genomic characteristics, we propose the genus Raoultibacter gen. nov., which contains strains Marseille‐P2849 T (= CSUR P2849 T , = DSM 103407 T ) and Marseille‐P3277 T (=CCUG 70680 T , =CSUR P3277 T ) as type strains of the species R. massiliensis sp. nov., and R. timonensis sp. nov., respectively.