Open AccessGenetic diversity and antimicrobial resistance in Staphylococcus aureus and coagulase‐negative Staphylococcus isolates from bovine mastitis in Minas Gerais, Brazil
Author(s) -
Dorneles Elaine M. S.,
Fonseca Mariana D. A. M.,
Abreu Juliana A. P.,
Lage Andrey P.,
Brito Maria A. V. P.,
Pereira Carine R.,
Brandão Humberto M.,
Guimarães Alessandro S.,
Heinemann Marcos B.
Publication year - 2019
Publication title -
microbiologyopen
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.881
H-Index - 36
ISSN - 2045-8827
DOI - 10.1002/mbo3.736
Subject(s) - pulsed field gel electrophoresis , staphylococcus aureus , coagulase , microbiology and biotechnology , mastitis , biology , antibiotic resistance , staphylococcus , antimicrobial , veterinary medicine , genetic diversity , genotype , antibiotics , bacteria , medicine , genetics , population , environmental health , gene
The aims of this study were to determine the antimicrobial susceptibility profile and genetic diversity of Staphylococcus spp. isolated from dairy cows in Minas Gerais, Brazil, and to assess the relationship among the isolates’ susceptibility profiles and pulsed‐field gel electrophoresis ( PFGE ) genotypes. Seventy‐nine isolates were used, including S. aureus ( n = 71) and coagulase‐negative staphylococci (Co NS ) ( n = 8). Susceptibility to 12 antimicrobial agents was performed. All Staphylococcus spp. were subjected to PFGE . Staphylococcus aureus and Co NS isolates exhibited full susceptibility only to cephalothin. The greatest percentages of resistance among Staphylococcus spp. were observed to penicillins, folate pathway inhibitors, and tetracyclines. Twelve S. aureus and four Co NS were classified as multidrug resistance strains. Percentage of MRSA was also higher among Co NS (75%), compared to S. aureus isolates (2.81%). Adopting 100% of similarity, 34 different genotypes were identified. Association of minimum‐spanning tree ( MST ) analysis with data from municipalities, herds, methicillin‐resistant S. aureus ( MRSA ), and resistance patterns for all isolates did not show any clustering. However, a clustering pattern of bacterial species was observed. Results from this study indicate a high frequency of antimicrobial resistance, especially among Co NS , and a high genetic diversity among Staphylococcus spp. isolated from dairy cows with mastitis in Minas Gerais, Brazil.