Open AccessW361R mutation in GaaR, the regulator of D‐galacturonic acid‐responsive genes, leads to constitutive production of pectinases in Aspergillus niger
Author(s) -
Alazi Ebru,
Niu Jing,
Otto Simon B.,
Arentshorst Mark,
Pham Thi T. M.,
Tsang Adrian,
Ram Arthur F. J.
Publication year - 2019
Publication title -
microbiologyopen
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.881
H-Index - 36
ISSN - 2045-8827
DOI - 10.1002/mbo3.732
Subject(s) - aspergillus niger , inducer , repressor , biochemistry , gene , biology , gene cluster , microbiology and biotechnology , gene expression
Polysaccharides present in plant biomass, such as pectin, are the main carbon source for filamentous fungi. Aspergillus niger naturally secretes pectinases to degrade pectin and utilize the released monomers, mainly D‐galacturonic acid. The transcriptional activator GaaR, the repressor of D‐galacturonic acid utilization GaaX, and the physiological inducer 2‐keto‐3‐deoxy‐L‐galactonate play important roles in the transcriptional regulation of D‐galacturonic acid‐responsive genes, which include the genes encoding pectinases. In this study, we described the mutations found in gaaX and gaaR that enabled constitutive (i.e., inducer‐independent) expression of pectinases by A. niger . Using promoter‐reporter strains (P pgaX ‐ amdS ) and polygalacturonic acid plate assays, we showed that W361R mutation in GaaR results in constitutive production of pectinases. Analysis of subcellular localization of C‐terminally eGFP ‐tagged GaaR/Gaa R W 361R revealed important differences in nuclear accumulation of N‐ versus C‐terminally eGFP ‐tagged GaaR.