
Wolbachia pipientis grows in Saccharomyces cerevisiae evoking early death of the host and deregulation of mitochondrial metabolism
Author(s) -
UribeAlvarez Cristina,
ChiqueteFélix Natalia,
MoralesGarcía Lilia,
BohórquezHernández Arlette,
DelgadoBuenrostro Norma Laura,
Vaca Luis,
Peña Antonio,
UribeCarvajal Salvador
Publication year - 2019
Publication title -
microbiologyopen
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.881
H-Index - 36
ISSN - 2045-8827
DOI - 10.1002/mbo3.675
Subject(s) - wolbachia , saccharomyces cerevisiae , biology , host (biology) , yeast , insect , microbiology and biotechnology , genetics , ecology
Wolbachia sp. has colonized over 70% of insect species, successfully manipulating host fertility, protein expression, lifespan, and metabolism. Understanding and engineering the biochemistry and physiology of Wolbachia holds great promise for insect vector‐borne disease eradication. Wolbachia is cultured in cell lines, which have long duplication times and are difficult to manipulate and study. The yeast strain Saccharomyces cerevisiae W303 was used successfully as an artificial host for Wolbachia w A lbB. As compared to controls, infected yeast lost viability early, probably as a result of an abnormally high mitochondrial oxidative phosphorylation activity observed at late stages of growth. No respiratory chain proteins from Wolbachia were detected, while several Wolbachia F 1 F 0 ‐ ATP ase subunits were revealed. After 5 days outside the cell, Wolbachia remained fully infective against insect cells.