Open AccessBiological degradation of aflatoxin M 1 by Bacillus pumilus E‐1‐1‐1
Author(s) -
Gu Xinxi,
Sun Jilu,
Cui Yuqi,
Wang Xianghong,
Sang Yaxin
Publication year - 2019
Publication title -
microbiologyopen
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.881
H-Index - 36
ISSN - 2045-8827
DOI - 10.1002/mbo3.663
Subject(s) - bacillus pumilus , strain (injury) , atomic force microscopy , degradation (telecommunications) , aflatoxin , 16s ribosomal rna , chemistry , food science , microbiology and biotechnology , materials science , bacteria , biology , nanotechnology , gene , biochemistry , telecommunications , genetics , computer science , anatomy
Aflatoxin M 1 (AFM 1 ) is a potent mycotoxin which causes serious health concerns in developing countries, where it is mainly found in milk, meat, and other foods. Biological detoxification is a promising method for eliminating AFM 1 . The aim of this work was to search for AFM 1 ‐degrading bacterial strains from animal waste, soil, and activated sludge. High‐performance liquid chromatography and Fourier‐transform infrared spectroscopy were used to analyze the AFM 1 degradation products. A strain designated E‐1‐1‐1 was obtained from African elephants feces, with the degradation ratio of AFM 1 reaching 89.55% in 12 hr. Based on morphology, physiological and biochemical tests, and 16S rRNA gene sequence analysis, strain E‐1‐1‐1 was identified as Bacillus pumilus . The culture supernatant of B. pumilus E‐1‐1‐1 degraded AFM 1 effectively, whereas the cells and cell extracts of B. pumilus E‐1‐1‐1 were far less effective. Carbon and nitrogen sources had highly significant effects on the degradation of AFM 1 by B. pumilus E‐1‐1‐1. The AFM 1 ‐degrading strain, B. pumilus E1‐1‐1, could have great potential in industrial applications.