Open AccessComparative genomic analysis of Myroides odoratimimus isolates
Author(s) -
Hu Shaohua,
Cao Lin,
Wu Yiyin,
Zhou Yajun,
Jiang Tao,
Wang Liqiang,
Wang Qiujing,
Ming Desong,
Chen Shicheng,
Wang Mingxi
Publication year - 2019
Publication title -
microbiologyopen
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.881
H-Index - 36
ISSN - 2045-8827
DOI - 10.1002/mbo3.634
Subject(s) - biology , genome , prophage , microbiology and biotechnology , pathogen , antibiotics , antibiotic resistance , virulence , gene , comparative genomics , genetics , genomics , bacteriophage , escherichia coli
Myroides odoratimimus is an important nosocomial pathogen. Management of M. odoratimimus infection is difficult owing to the multidrug resistance and the unknown pathogenesis mechanisms. Based on our previous genomic sequencing data of M. odoratimimus PR63039 (isolated from a patient with the urinary tract infection), in this study, we further performed comparative genomic analysis for 10 selected Myroides strains. Our results showed that these Myroides genome contexts were very similar and phylogenetically related. Various prophages were identified in the four clinical isolate genomes, which possibly contributed to the genome evolution among the Myroides strains. CRISPR elements were only detected in the two clinical ( PR 63039 and CCUG 10230) isolates and two environmental ( CCUG 12700 and H1bi) strains. With more stringent cutoff parameters in CARD analysis, the four clinical M. odoratimimus contained roughly equal antibiotic resistance genes, indicating their similar antibiotic resistance profiles. The three clinical ( CCUG 10230, CCUG 12901, CIP 101113) and three environmental ( CCUG 12700, L41, H1bi) M. odoratimimus strains were speculated to carry the indistinguishable virulent factors ( VF s), which may involve in the similar pathogenesis mechanism. Moreover, some VF s might confer to the high capacity of dissemination, attacking tissue cells and induction of autoimmune complications. Our results facilitate the research of antibiotic resistance and the development of therapeutic regimens for the M. odoratimimus infections.