Open AccessSusceptibilities of Candidatus Liberibacter asiaticus‐infected and noninfected Diaphorina citri to entomopathogenic fungi and their detoxification enzyme activities under different temperatures
Author(s) -
Hussain Mubasher,
Akutse Komivi Senyo,
Lin Yongwen,
Chen Shiman,
Huang Wei,
Zhang Jinguan,
Idrees Atif,
Qiu Dongliang,
Wang Liande
Publication year - 2018
Publication title -
microbiologyopen
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.881
H-Index - 36
ISSN - 2045-8827
DOI - 10.1002/mbo3.607
Subject(s) - diaphorina citri , entomopathogenic fungi , biology , esterase , entomopathogenic fungus , hemiptera , microbiology and biotechnology , botany , veterinary medicine , biological pest control , enzyme , beauveria bassiana , biochemistry , medicine
Some entomopathogenic fungi species, Isaria fumosorosea , and Hirsutella citriformis were found to be efficient against the Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Liviidae). However, the susceptibility to these fungi increases when the psyllid infected with Candidatus Liberibacter asiaticus (Las), which is transmitted by D. citri and causes citrus greening disease. In this study, we examined the Las‐infected and Las‐uninfected D . citri susceptibility to entomopathogenic fungi at different temperature regimes (5–40°C). When D. citri adults exposed to cold temperature (5°C), they showed less susceptibility to entomopathogenic fungi as compared with control (27°C). Irrespective of infection with Las, a significantly positive correlation was observed between temperature and percentage mortality caused by different isolates of I. fumosorosea , 3A Ifr , 5F Ifr , PS Ifr , and H. citriformis isolates, HC 3D and 2H. In contrast, a significantly negative correlation was found between temperature and percentage mortality for 3A Ifr for both Las‐infected and Las‐uninfected psyllids. Detoxification enzymes, Glutathione S‐transferase levels in D . citri showed a negative correlation, whereas cytochrome P450 and general esterase levels were not correlated with changes in temperature. These findings revealed that detoxification enzymes and general esterase levels are not correlated with altered susceptibility to entomopathogenic fungi at the different temperature regimes. Conclusively, temperature fluctuations tested appear to be a significant factor impacting the management strategies of D. citri using entomopathogenic fungi.