Open AccessCharacterization of a novel Gram‐stain‐positive anaerobic coccus isolated from the female genital tract: Genome sequence and description of Murdochiella vaginalis sp. nov.
Author(s) -
Diop Khoudia,
Diop Awa,
Khelaifia Saber,
Robert Catherine,
Pinto Fabrizio Di,
Delerce Jérémy,
Raoult Didier,
Fournier PierreEdouard,
Bretelle Florence,
Fenollar Florence
Publication year - 2018
Publication title -
microbiologyopen
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.881
H-Index - 36
ISSN - 2045-8827
DOI - 10.1002/mbo3.570
Subject(s) - coccus (insect) , 16s ribosomal rna , biology , strain (injury) , microbiology and biotechnology , gram staining , whole genome sequencing , gene , genome , genetics , anatomy , botany , antibiotics
Strain Marseille‐P2341 T , a nonmotile, nonspore‐forming, Gram‐stain‐positive anaerobic coccus, was isolated in the vaginal specimen of a patient with bacterial vaginosis using culturomics. Its growth occurred at temperatures ranging from 25 to 42°C, with pH between 6.5 and 8.5, and at NaCl concentrations lower than 5%. The major fatty acids were C 18:1n9 (27.7%) and C 16:0 (24.4%). Its genome is 1,671,491 bp long with 49.48 mol% of G+C content. It is composed of 1,501 genes: 1,446 were protein‐coding genes and 55 were RNA s. Strain Marseille‐P2341 T shared 97.3% of 16S rRNA gene sequence similarity with Murdochiella asaccharolytica , the phylogenetically closest species. These results enabled the classification of strain Marseille‐P2341 T as a new species of the genus Murdochiella for which we proposed the name Murdochiella vaginalis sp. nov. The type strain is strain Marseille‐P2341 T (= DSM 102237, = CSUR P2341).