Open AccesssecA , secD , secF , yajC, and yidC contribute to the adhesion regulation of Vibrio alginolyticus
Author(s) -
Guo Lina,
Huang Lixing,
Su Yongquan,
Qin Yingxue,
Zhao Lingmin,
Yan Qingpi
Publication year - 2018
Publication title -
microbiologyopen
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.881
H-Index - 36
ISSN - 2045-8827
DOI - 10.1002/mbo3.551
Subject(s) - vibrio alginolyticus , adhesion , gene silencing , secretion , virulence , microbiology and biotechnology , biology , gene , chemistry , biochemistry , vibrio , genetics , bacteria , organic chemistry
Vibrio alginolyticus caused great losses to aquaculture. Adhesion is an important virulence factor of V. alginolyticus . In this study, the relationship between V. alginolyticus adhesion and type II secretion system genes ( secA , secD , secF , yajC, and yidC ) was determined using gene silencing, qRT ‐ PCR and in vitro adhesion assay. The results showed that the expression of target genes and the bacterial adhesion exhibited significant decreases after transient gene silencing and stable gene silencing, which indicated that secA , secD , secF , yajC, and yidC played roles in the bacterial adhesion of V. alginolyticus . The expression of secA , secD , secF , yajC, and yidC were significantly influenced by temperature, salinity, pH and starvation. The results indicated that the expression of secA , secD , secF , yajC, and yidC were sensitive to different environmental factors, whereas environmental factors can affect V. alginolyticus adhesion via the expression of secA , secD , secF , yajC, and yidC .