Open AccessBacillus kwashiorkori sp. nov., a new bacterial species isolated from a malnourished child using culturomics
Author(s) -
Seck El hadji,
Beye Mamadou,
Traore Sory Ibrahima,
Khelaifia Saber,
Michelle Caroline,
Couderc Carine,
Brah Souleymane,
Fournier PierreEdouard,
Raoult Didier,
Bittar Fadi
Publication year - 2018
Publication title -
microbiologyopen
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.881
H-Index - 36
ISSN - 2045-8827
DOI - 10.1002/mbo3.535
Subject(s) - 16s ribosomal rna , biology , contig , gene , strain (injury) , microbiology and biotechnology , ribosomal rna , genome , oxidase test , whole genome sequencing , genomic dna , bacteria , genetics , biochemistry , anatomy , enzyme
Strain SIT 6 T was isolated from the fecal flora of a severely malnourished child as part of a broad “culturomics” study aiming to maximize the culture conditions for the in‐depth exploration of the human microbiota. An analysis of the 16S rRNA gene sequence showed that strain SIT 6 T shared 94.1% 16S rRNA gene sequence similarity with Bacillus thermoamylovorans DKP T ( NR _029151), the phylogenetically closest type species. Colonies are creamy white, circular, 4–5 mm in diameter after cultivation at 37°C for 24 hr on 5% sheep blood‐enriched Colombia agar. Growth occurs at temperatures in the range of 25–56°C (optimally at 37°C). Strain SIT 6 T is a gram‐positive, facultative anaerobic rod and motile by means of peritrichous flagella and sporulating; it is catalase and oxidase positive. The 2,784,637‐bp‐long genome, composed of 16 contigs, has a G+C content of 35.19%. Of the 2,646 predicted genes, 2,572 were protein‐coding genes and 74 were RNA s. The major fatty acids are saturated species (15:0 iso, 16:0 and 17:0 anteiso). Of the 14 detected fatty acids, 11 are saturated, either linear or branched (iso and anteiso). Digital DNA – DNA hybridization ( dDDH ) estimation and average genomic identity of orthologous gene sequences ( AGIOS ) of the strain SIT 6 T against genomes of the type strains of related species ranged between 18.6% and 38.3% and between 54.77% and 65.50%, respectively. According to our taxonogenomics results, we propose the creation of Bacillus kwashiorkori sp. nov. that contains the type strain SIT 6 T (= CSUR P2452 T , = DSM 29059 T ).