Open AccessThe Lactococcus lactis KF 147 nonribosomal peptide synthetase/polyketide synthase system confers resistance to oxidative stress during growth on plant leaf tissue lysate
Author(s) -
Golomb Benjamin L.,
Yu Annabelle O.,
Coates Laurynne C.,
Marco Maria L.
Publication year - 2018
Publication title -
microbiologyopen
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.881
H-Index - 36
ISSN - 2045-8827
DOI - 10.1002/mbo3.531
Subject(s) - nonribosomal peptide , polyketide synthase , biology , lactococcus lactis , biochemistry , polyketide , lysis , mutant , bacteria , microbiology and biotechnology , gene , biosynthesis , genetics , lactic acid
Strains of Lactococcus lactis isolated from plant tissues possess adaptations that support their survival and growth in plant‐associated microbial habitats. We previously demonstrated that genes coding for a hybrid nonribosomal peptide synthetase/polyketide synthase ( NRPS / PKS ) system involved in production of an uncharacterized secondary metabolite are specifically induced in L. lactis KF 147 during growth on plant tissues. Notably, this NRPS / PKS has only been identified in plant‐isolated strains of L. lactis . Here, we show that the L. lactis KF 147 NRPS / PKS genes have homologs in certain Streptococcus mutans isolates and the genetic organization of the NRPS / PKS locus is conserved among L. lactis strains. Using an L. lactis KF 147 mutant deficient in synthesis of NrpC, a 4′‐phosphopantetheinyl transferase, we found that the NRPS / PKS system improves L. lactis during growth under oxidative conditions in Arapidopsis thaliana leaf lysate. The NRPS / PKS system also improves tolerance of L. lactis to reactive oxygen species and specifically H 2 O 2 and superoxide radicals in culture medium. These findings indicate that this secondary metabolite provides a novel mechanism for reactive oxygen species detoxification not previously known for this species.