Open AccessComplete characterization of new isolates of Neptunomonas phycophila leads to emend its description and opens possibilities of biotechnological applications
Author(s) -
Diéguez Ana L.,
Pichon Phillip,
Balboa Sabela,
Magnesen Thorolf,
Romalde Jesús L.
Publication year - 2017
Publication title -
microbiologyopen
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.881
H-Index - 36
ISSN - 2045-8827
DOI - 10.1002/mbo3.519
Subject(s) - biology , 16s ribosomal rna , strain (injury) , scallop , genome , botany , housekeeping gene , gene , pecten maximus , genome size , genetics , zoology , ecology , anatomy , gene expression , mollusca , bivalvia
Five strains were isolated from gonad of Great scallop ( Pecten maximus ) broodstock in a Norwegian hatchery. The study of 16S rRNA gene sequences showed that these isolates belong to Neptunomonas phycophila , a bacterium originally isolated from a symbiont of the anemone Aiptasia tagetes from Puerto Rico. The gyr B and rpo B genes sequences confirmed the affiliation of the scallop isolates to this species. Phenotypic characterization was performed and some differences between the Norwegian isolates and the type strain of N. phycophila were detected, such as ranges of temperature, pH , and tolerance to salinity or the use of several substrates as sole carbon source which lead to an emended description of the species. The strain 3 CM 2.5 showed phosphatidylethanolamine and phosphatidylglycerol as the major polar lipids. The whole genomes of the scallop strain 3 CM 2.5 and type strain of the species CECT 8716 T were obtained and the annotation of these genomes revealed the presence of genes involved in degradation of aromatic compounds in both strains. Results obtained not only widen the geographical and host ranges of N. phycophila , but also point out possible biotechnological applications for this bacterial species.