Open AccesswblE2 transcription factor in Streptomyces griseus S4‐7 plays an important role in plant protection
Author(s) -
Cho Hyun Ji,
Kwon Young Sang,
Kim DaRan,
Cho Gyeongjun,
Hong Seong Won,
Bae DongWon,
Kwak YounSig
Publication year - 2017
Publication title -
microbiologyopen
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.881
H-Index - 36
ISSN - 2045-8827
DOI - 10.1002/mbo3.494
Subject(s) - streptomyces griseus , biology , fusarium oxysporum , fusarium wilt , mutant , transcription factor , microbiology and biotechnology , secondary metabolism , streptomyces , rhizosphere , transcriptome , polyketide , gene , botany , genetics , gene expression , bacteria , biosynthesis
Streptomyces griseus S4‐7 was originally isolated from the strawberry rhizosphere as a microbial agent responsible for Fusarium wilt suppressive soils. S. griseus S4‐7 shows specific and pronounced antifungal activity against Fusarium oxysporum f. sp. fragariae . In the Streptomyces genus, the whi transcription factors are regulators of sporulation, cell differentiation, septation, and secondary metabolites production. wblE2 function as a regulator has emerged as a new group in whi transcription factors. In this study, we reveal the involvement of the wblE2 transcription factor in the plant‐protection by S. griseus S4‐7. We generated Δ wblE , Δ wblE2 , Δ whiH , and Δ whmD gene knock‐out mutants, which showed less antifungal activity both in vitro and in planta . Among the mutants, wblE 2 mutant failed to protect the strawberry against the Fusarium wilt pathogen. Transcriptome analyses revealed major differences in the regulation of phenylalanine metabolism, polyketide and siderophore biosynthesis between the S4‐7 and the wblE 2 mutant. The results contribute to our understanding of the role of streptomycetes wblE2 genes in a natural disease suppressing system.