Open AccessTranscriptional regulation of cpsQ ‐ mfp ABC and mfp ABC by CalR in Vibrio parahaemolyticus
Author(s) -
Gao He,
Zhang Lingyu,
OseiAdjei George,
Yang Wenhui,
Zhou Dongsheng,
Huang Xinxiang,
Yang Huiying,
Yin Zhe,
Zhang Yiquan
Publication year - 2017
Publication title -
microbiologyopen
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.881
H-Index - 36
ISSN - 2045-8827
DOI - 10.1002/mbo3.470
Subject(s) - atp binding cassette transporter , operon , repressor , vibrio parahaemolyticus , biology , promoter , transcription (linguistics) , genetics , gene , microbiology and biotechnology , transcription factor , gene expression , bacteria , transporter , mutant , linguistics , philosophy
The cpsQ‐mfp ABC locus is transcribed as two operons, i.e., cpsQ‐mfp ABC and mfp ABC , in Vibrio parahaemolyticus , and both of them are all required for biofilm formation. CalR belongs to the LysR‐type transcriptional regulator family, and was originally identified as a repressor of the swarming motility and T3 SS 1 genes expression in V . parahaemolyticus . In the present work, a combination of qRT ‐ PCR , primer extension, LacZ fusion expression, electrophoretic mobility shift assay, and DN ase I footprinting assays were employed to elucidate the regulatory mechanisms of cpsQ‐mfp ABC and mfp ABC by CalR. One transcription start site for each operon was detected and their activities were activated by CalR. His‐CalR protected two DNA regions upstream of mfp ABC against DN ase I digestion, but no binding sites were detected in the promoter region of cpsQ‐mfp ABC , suggesting a direct and an indirect regulatory manner for mfp ABC and cpsQ‐mfp ABC transcription by CalR, respectively. Collectively, the results presented here confirmed a new physiological role for CalR that acts as an activator for cpsQ‐mfp ABC and mfp ABC transcription.