Open AccessA novel regulator RcdA of the csgD gene encoding the master regulator of biofilm formation in E scherichia coli
Author(s) -
Shimada Tomohiro,
Katayama Yasunori,
Kawakita Shuichi,
Ogasawara Hiroshi,
Nakano Masahiro,
Yamamoto Kaneyoshi,
Ishihama Akira
Publication year - 2012
Publication title -
microbiologyopen
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.881
H-Index - 36
ISSN - 2045-8827
DOI - 10.1002/mbo3.42
Subject(s) - regulator , tetr , dna , protein–dna interaction , transcription factor , gene , transcription (linguistics) , dna footprinting , biology , transcriptional regulation , systematic evolution of ligands by exponential enrichment , microbiology and biotechnology , cooperativity , promoter , dna binding protein , genetics , gene expression , repressor , rna , linguistics , philosophy
The FixJ / LuxR family transcription factor CsgD is a master regulator of biofilm formation in E scherichia coli . Previously, we identified more than 10 transcription factors that participate in regulation of the csgD promoter. After genomic SELEX screening of regulation targets, an uncharacterized TetR ‐type transcription factor YbjK was found to be involved in regulation of the csgD promoter. In addition, a number of stress‐response genes were found to be under the direct control of YbjK . Taken together, we propose to rename it to RcdA (regulator of csgD ). One unique feature of RcdA is its mode of DNA binding. Gel shift, DNase‐I footprinting, and atomic force microscopic ( AFM ) analyses indicated that RcdA is a DNA ‐binding protein with a high level of cooperativity, with which it covers the entire surface of probe DNA through protein–protein interaction and moreover it induces the formation of aggregates of DNA – RcdA complexes.