Open AccessAgarolytic bacterium Persicobacter sp. CCB ‐ QB 2 exhibited a diauxic growth involving galactose utilization pathway
Author(s) -
Furusawa Go,
Lau NyokSean,
Suganthi Appalasamy,
Amirul Abdullah AlAshraf
Publication year - 2017
Publication title -
microbiologyopen
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.881
H-Index - 36
ISSN - 2045-8827
DOI - 10.1002/mbo3.405
Subject(s) - bacteria , galactose , galactokinase , biology , biochemistry , phosphoglucomutase , microbiology and biotechnology , enzyme , gene , escherichia coli , genetics
The agarolytic bacterium Persicobacter sp. CCB ‐ QB 2 was isolated from seaweed (genus Ulva ) collected from a coastal area of Malaysia. Here, we report a high‐quality draft genome sequence for QB 2. The Rapid Annotation using Subsystem Technology ( RAST ) annotation server identified four β‐agarases (PdAgaA, PdAgaB, PdAgaC, and PdAgaD) as well as galK, galE, and phosphoglucomutase, which are related to the Leloir pathway. Interestingly, QB 2 exhibited a diauxic growth in the presence of two kinds of nutrients, such as tryptone and agar. In cells grown with agar, the profiles of agarase activity and growth rate were very similar. galK, galE, and phosphoglucomutase genes were highly expressed in the second growth phase of diauxic growth, indicating that QB 2 cells use galactose hydrolyzed from agar by its agarases and exhibit nutrient prioritization. This is the first report describing diauxic growth for agarolytic bacteria. QB 2 is a potential novel model organism for studying diauxic growth in environmental bacteria.