Open AccessIsolation of Bdellovibrio sp. from soil samples in Mexico and their potential applications in control of pathogens
Author(s) -
Oyedara Omotayo Opemipo,
De LunaSantillana Erick de Jesus,
OlguinRodriguez Omar,
Guo Xianwu,
MendozaVilla Marco Antonio,
MenchacaArredondo Jorge Luis,
Elufisan Temidayo Oluyomi,
GarzaHernandez Javier Alfonso,
Garcia Leon Israel,
RodriguezPerez Mario Alberto
Publication year - 2016
Publication title -
microbiologyopen
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.881
H-Index - 36
ISSN - 2045-8827
DOI - 10.1002/mbo3.382
Subject(s) - bdellovibrio , biology , microbiology and biotechnology , 16s ribosomal rna , isolation (microbiology) , bacteria , salmonella , enterobacteriaceae , genetics , gene , escherichia coli
In this study, two strains of Bdellovibrio were isolated from soil samples using the culture‐dependent technique and two members of the family Enterobacteriaceae ( Klebsiella sp. and Salmonella sp.) as prey. The Bdellovibrio strains were bacteriolytic, plaque‐forming, and highly motile gram‐negative bacteria. We identified and confirmed the Bdellovibrio strains using microscopy, PCR amplification, and sequencing of the 16S rRNA gene. They were observed to be different strains based on hit locus and prey range analyses. Here, the first report on Bdellovibrio strains isolated from soil in Mexico corroborates earlier report indicating that populations of Bdellovibrio found in soil are heterogeneous thereby the need to identify the various strains.