Open AccessAdaptive responses of outer membrane porin balance of Yersinia ruckeri under different incubation temperature, osmolarity, and oxygen availability
Author(s) -
Bystritskaya Evgeniya,
Stenkova Anna,
Chistuylin Dmitriy,
Chernysheva Nadezhda,
Khomenko Valentina,
Anastyuk Stanislav,
Novikova Olga,
Rakin Alexander,
Isaeva Marina
Publication year - 2016
Publication title -
microbiologyopen
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.881
H-Index - 36
ISSN - 2045-8827
DOI - 10.1002/mbo3.354
Subject(s) - porin , yersinia ruckeri , osmotic concentration , biology , bacterial outer membrane , microbiology and biotechnology , osmotic shock , escherichia coli , incubation , biochemistry , gene , fishery , fish <actinopterygii> , rainbow trout
Abstract The capability of Yersinia ruckeri to survive in the aquatic systems reflects its adaptation (most importantly through the alteration of membrane permeability) to the unfavorable environments. The nonspecific porins are a key factor contributing to the permeability. Here we studied the influence of the stimuli, such as temperature, osmolarity, and oxygen availability on regulation of Y. ruckeri porins. Using qRT ‐ PCR and SDS ‐ PAGE methods we found that major porins are tightly controlled by temperature. Hyperosmosis did not repress OmpF production. The limitation of oxygen availability led to decreased expression of both major porins and increased transcription of the minor porin OmpY. Regulation of the porin balance in Y. ruckeri , in spite of some similarities, diverges from that system in Escherichia coli . The changes in porin regulation can be adapted in Y. ruckeri in a species‐specific manner determined by its aquatic habitats.