Open AccessIron‐regulated metabolites produced by P seudomonas fluorescens WCS 374r are not required for eliciting induced systemic resistance against P seudomonas syringae pv. tomato in A rabidopsis
Author(s) -
Djavaheri Mohammad,
MercadoBlanco Jesús,
Versluis C.,
Meyer J.M.,
Loon L. C.,
Bakker Peter A. H. M.
Publication year - 2012
Publication title -
microbiologyopen
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.881
H-Index - 36
ISSN - 2045-8827
DOI - 10.1002/mbo3.32
Subject(s) - siderophore , pseudomonas fluorescens , mutant , biology , pseudomonas syringae , mutagenesis , microbiology and biotechnology , salicylic acid , arabidopsis thaliana , heterologous , transposable element , biochemistry , bacteria , gene , genetics , pathogen
The plant growth‐promoting rhizobacterium P seudomonas fluorescens WCS 374r produces several iron‐regulated metabolites, including the fluorescent siderophore pseudobactin (Psb374), salicylic acid ( SA ), and pseudomonine (Psm), a siderophore that contains a SA moiety. After purification of Psb374 from culture supernatant of WCS 374r, its structure was determined following isoelectrofocusing and tandem mass spectrometry, and found to be identical to the fluorescent siderophore produced by P . fluorescens ATCC 13525. To study the role of SA and Psm production in colonization of Arabidopsis thaliana roots and in induced systemic resistance ( ISR ) against P seudomonas syringae pv. tomato (Pst) by strain WCS 374r, mutants disrupted in the production of these metabolites were obtained by homologous recombination. These mutants were further subjected to transposon Tn 5 mutagenesis to generate mutants also deficient in Psb374 production. The mutants behaved similar to the wild type in both their A rabidopsis rhizosphere‐colonizing capacity and their ability to elicit ISR against Pst. We conclude that Psb374, SA , and Psm production by P . fluorescens WCS 374r are not required for eliciting ISR in A rabidopsis.