Open AccessA factor converting viable but nonculturable Vibrio cholerae to a culturable state in eukaryotic cells is a human catalase
Author(s) -
Senoh Mitsutoshi,
Hamabata Takashi,
Takeda Yoshifumi
Publication year - 2015
Publication title -
microbiologyopen
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.881
H-Index - 36
ISSN - 2045-8827
DOI - 10.1002/mbo3.264
Subject(s) - vibrio cholerae , viable but nonculturable , catalase , microbiology and biotechnology , gene knockdown , vibrionaceae , bacteria , biology , enzyme , chemistry , biochemistry , gene , genetics
In our previous work, we demonstrated that viable but nonculturable ( VBNC ) Vibrio cholerae O1 and O139 were converted to culturable by coculture with eukaryotic cells. Furthermore, we isolated a factor converting VBNC V. cholerae to culturable ( FCVC ) from a eukaryotic cell line, HT ‐29. In this study, we purified FCVC by successive column chromatographies comprising UNO Q‐6 anion exchange, Bio‐Scale CHT 2‐1 hydroxyapatite, and Superdex 200 10/300 GL. Homogeneity of the purified FCVC was demonstrated by SDS ‐ PAGE . Nano‐ LC MS / MS analysis showed that the purified FCVC was a human catalase. An experiment of RNA i knockdown of catalase mRNA from HT ‐29 cells and treatment of the purified FCVC with a catalase inhibitor, 3‐amino‐1,2,4‐triazole confirmed that the FCVC was a catalase. A possible role of the catalase in converting a VBNC V. cholerae to a culturable state in the human intestine is discussed.