Open AccessA highly acid‐resistant novel strain of Lactobacillus johnsonii No. 1088 has antibacterial activity, including that against Helicobacter pylori, and inhibits gastrin‐mediated acid production in mice
Author(s) -
Aiba Yuji,
Nakano Yasuhiro,
Koga Yasuhiro,
Takahashi Kenji,
Komatsu Yasuhiko
Publication year - 2015
Publication title -
microbiologyopen
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.881
H-Index - 36
ISSN - 2045-8827
DOI - 10.1002/mbo3.252
Subject(s) - microbiology and biotechnology , helicobacter pylori , biology , strain (injury) , bacteria , escherichia coli , gastric acid , gastrin , lactobacillus , secretion , virulence , biochemistry , gene , genetics , anatomy
A novel strain of Lactobacillus johnsonii No. 1088 was isolated from the gastric juice of a healthy Japanese male volunteer, and characterized for its effectiveness in the stomach environment. Lactobacillus johnsonii No. 1088 was found to have the strongest acid resistance among several lactobacilli examined (>10% of cells survived at pH 1.0 after 2 h), and such a high acid resistance property was a specific characteristic of this strain of L. johnsonii . When cultured with various virulent bacteria, L. johnsonii No. 1088 inhibited the growth of Helicobacter pylori , Escherichia coli O‐157, Salmonella Typhimurium, and Clostridium difficile , in which case its effectiveness was more potent than that of a type strain of L. johnsonii, JCM 2012. In addition to its effect in vitro, L. johnsonii No. 1088 inhibited the growth of H. pylori in human intestinal microbiota‐associated mice in both its live and lyophilized forms. Moreover, L. johnsonii No. 1088 suppressed gastric acid secretion in mice via decreasing the number of gastrin‐positive cells in the stomach. These results taken together suggest that L. johnsonii No. 1088 is a unique lactobacillus having properties beneficial for supporting H. pylori eradication by triple therapy including the use of a proton pump inhibitor ( PPI ) and also for prophylaxis of gastroesophageal reflux disease possibly caused after H. pylori eradication as a side effect of PPI .