Open AccessMicrobial diversity and abundance in the Xinjiang Luliang long‐term water‐flooding petroleum reservoir
Author(s) -
Gao Peike,
Tian Huimei,
Li Guoqiang,
Sun Hongwen,
Ma Ting
Publication year - 2015
Publication title -
microbiologyopen
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.881
H-Index - 36
ISSN - 2045-8827
DOI - 10.1002/mbo3.241
Subject(s) - biology , methanosaeta , halomonas , arcobacter , microbiology and biotechnology , chloroflexi (class) , proteobacteria , microbial population biology , 16s ribosomal rna , bacteria , genetics
Microbial populations associated with microbial enhanced oil recovery ( MEOR ) and their abundance in the Xinjiang Luliang water‐flooding petroleum reservoir were investigated using 16S rRNA , nitrate reductases, dissimilatory sulfate reductase, and methyl coenzyme‐M reductase‐encoded genes to provide ecological information for the potential application of MEOR . 16S rRNA gene miseq sequencing revealed that this reservoir harbored large amounts of taxa, including 155 bacterial and 7 archeal genera. Among them, Arcobacter , Halomonas , Marinobacterium , Marinobacter , Sphingomonas , Rhodococcus , Pseudomonas , Dietzia , Ochrobactrum , Hyphomonas , Acinetobacter, and Shewanella were dominant, and have the potential to grow using hydrocarbons as carbon sources. Metabolic gene clone libraries indicated that the nitrate‐reducing bacteria ( NRB ) mainly belonged to Pseudomonas , Azospirillum , Bradyrhizobium , Thauera , Magnetospirillum , Sinorhizobium , Azoarcus , and Rhodobacter ; the sulfate‐reducing bacteria ( SRB ) were Desulfarculus , Desulfomonile , Desulfosarcina , Desulfotignum , Desulfacinum , Desulfatibacillum , Desulfatibacillum , Desulfomicrobium , and Desulfovibrio ; while the methanogens were archaea and belonged to Methanomethylovorans , Methanosaeta , Methanococcus , Methanolobus , and Methanobacterium . Real‐time quantitative PCR analysis indicated that the number of bacterial 16S rRNA reached 10 6 copies/mL, while the metabolic genes of NRB , SRB , and methanogens reached 10 4 copies/mL. These results show that the Luliang reservoir has abundant microbial populations associated with oil recovery, suggesting that the reservoir has potential for MEOR.