Open AccessColony morphology and transcriptome profiling of P seudomonas putida KT 2440 and its mutants deficient in alginate or all EPS synthesis under controlled matric potentials
Author(s) -
Gulez Gamze,
Altıntaş Ali,
Fazli Mustafa,
Dechesne Arnaud,
Workman Christopher T.,
TolkerNielsen Tim,
Smets Barth F.
Publication year - 2014
Publication title -
microbiologyopen
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.881
H-Index - 36
ISSN - 2045-8827
DOI - 10.1002/mbo3.180
Subject(s) - pseudomonas putida , mutant , bacterial cellulose , chemistry , polysaccharide , transcriptome , wild type , biochemistry , biology , microbiology and biotechnology , cellulose , gene , gene expression
Pseudomonas putida is a versatile bacterial species adapted to soil and its fluctuations. Like many other species living in soil, P. putida often faces water limitation. Alginate, an exopolysaccharide ( EPS ) produced by P. putida , is known to create hydrated environments and alleviate the effect of water limitation. In addition to alginate, P. putida is capable of producing cellulose ( bcs ), putida exopolysaccharide a ( pea ), and putida exopolysaccharide b ( peb ). However, unlike alginate, not much is known about their roles under water limitation. Hence, in this study we examined the role of different EPS components under mild water limitation. To create environmentally realistic water limited conditions as observed in soil, we used the Pressurized Porous Surface Model. Our main hypothesis was that under water limitation and in the absence of alginate other exopolysaccharides would be more active to maintain homeostasis. To test our hypothesis, we investigated colony morphologies and whole genome transcriptomes of P. putida KT 2440 wild type and its mutants deficient in synthesis of either alginate or all known EPS . Overall our results support that alginate is an important exopolysaccharide under water limitation and in the absence of alginate other tolerance mechanisms are activated.