Open AccessImproved fluorescent Listeria spp. biosensors for analysis of antimicrobials by flow cytometry
Author(s) -
Reich Sebastian J.,
Stohr Jonas,
Goldbeck Oliver,
Fendrich Bastian,
Crauwels Peter,
Riedel Christian U.
Publication year - 2022
Publication title -
microbiologyopen
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.881
H-Index - 36
ISSN - 2045-8827
DOI - 10.1002/mbo3.1304
Subject(s) - flow cytometry , listeria monocytogenes , biosensor , listeria , antimicrobial , microbiology and biotechnology , microorganism , antibiotic resistance , biology , fluorescence , bacteria , antibiotics , chemistry , biochemistry , genetics , physics , quantum mechanics
The global increase in antibiotic resistance of pathogenic microorganisms requires the identification and characterization of novel antimicrobials. Bacterial biosensors expressing fluorescent proteins such as pHluorin variants are suitable for high‐throughput screenings. Here, we present Listeria spp. pH‐sensitive biosensors with improved fluorescence for single‐cell analysis of antimicrobials by flow cytometry.