Open AccessMetabolic profiling analysis of the vitamin B 12 producer Propionibacterium freudenreichii
Author(s) -
Liu Jiao,
Liu Yongfei,
Wu Jie,
Fang Huan,
Jin Zhaoxia,
Zhang Dawei
Publication year - 2021
Publication title -
microbiologyopen
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.881
H-Index - 36
ISSN - 2045-8827
DOI - 10.1002/mbo3.1199
Subject(s) - propionibacterium freudenreichii , biochemistry , metabolite , metabolism , propionibacterium , fermentation , metabolomics , citric acid cycle , yeast , metabolic pathway , chemistry , chemically defined medium , metabolome , vitamin b12 , biology , food science , chromatography , bacteria , in vitro , genetics
Vitamin B 12 (VB 12 ) is an indispensable cofactor of metabolic enzymes and has been widely used in the food and pharmaceutical industries. In this study, the effects of medium composition on VB 12 production by Propionibacterium freudenreichii were evaluated and optimized based on statistical experiments. The results showed that glucose, yeast extract, KH 2 PO 4 , and glycine have significant effects on VB 12 production. The final titer of VB 12 reached 8.32 ± 0.02 mg/L, representing a 120% increase over the non‐optimized culture medium. We employed a metabolomics approach to analyze the differences of metabolite concentrations in P . freudenreichii cells cultivated in the original medium and optimized fermentation medium. Using multivariate data analysis, we identified a range of correlated metabolites, illustrating how metabolomics can be used to explain VB 12 production changes by corresponding differences in the overall cellular metabolism. The concentrations of many metabolic intermediates of glycolysis, the Wood–Werkman cycle, the TCA cycle, and amino acid metabolism were increased, which contributed to the synthesis of propionic acid and VB 12 due to an improved supply of energy and precursors.