Open AccessMagnesium depletion extends fission yeast lifespan via general amino acid control activation
Author(s) -
Ohtsuka Hokuto,
Kobayashi Mikuto,
Shimasaki Takafumi,
Sato Teppei,
Akanuma Genki,
Kitaura Yasuyuki,
Otsubo Yoko,
Yamashita Akira,
Aiba Hirofumi
Publication year - 2021
Publication title -
microbiologyopen
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.881
H-Index - 36
ISSN - 2045-8827
DOI - 10.1002/mbo3.1176
Subject(s) - schizosaccharomyces pombe , yeast , polysome , amino acid , biology , biochemistry , microbiology and biotechnology , ribosome , nutrient sensing , longevity , gene , chemistry , genetics , saccharomyces cerevisiae , signal transduction , rna
Nutrients including glucose, nitrogen, sulfur, zinc, and iron are involved in the regulation of chronological lifespan (CLS) of yeast, which serves as a model of the lifespan of differentiated cells of higher organisms. Herein, we show that magnesium (Mg 2+ ) depletion extends CLS of the fission yeast Schizosaccharomyces pombe through a mechanism involving the Ecl1 gene family. We discovered that ecl1 + expression, which extends CLS, responds to Mg 2+ depletion. Therefore, we investigated the underlying intracellular responses. In amino acid auxotrophic strains, Mg 2+ depletion robustly induces ecl1 + expression through the activation of the general amino acid control (GAAC) pathway—the equivalent of the amino acid response of mammals. Polysome analysis indicated that the expression of Ecl1 family genes was required for regulating ribosome amount when cells were starved, suggesting that Ecl1 family gene products control the abundance of ribosomes, which contributes to longevity through the activation of the evolutionarily conserved GAAC pathway. The present study extends our understanding of the cellular response to Mg 2+ depletion and its influence on the mechanism controlling longevity.