Open AccessThe effect of sulfamonomethoxine treatment on the gut microbiota of Nile tilapia ( Oreochromis niloticus )
Author(s) -
Ming Junchao,
Fu Zhengyi,
Ma Zhenhua,
Zhou Lijun,
Zhang Zongli,
Song Chao,
Yuan Xinhua,
Wu Qinglong
Publication year - 2020
Publication title -
microbiologyopen
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.881
H-Index - 36
ISSN - 2045-8827
DOI - 10.1002/mbo3.1116
Subject(s) - nile tilapia , firmicutes , oreochromis , proteobacteria , biology , actinobacteria , tilapia , bacteroidetes , microbiology and biotechnology , food science , 16s ribosomal rna , bacteria , fish <actinopterygii> , fishery , genetics
To investigate the possible effects of sulfamonomethoxine (SMM) on Nile tilapia ( Oreochromis niloticus ), we quantitatively evaluated the microbial shifts in the intestines of Nile tilapia in response to different doses of SMM (200 and 300 mg/kg) using 16S rRNA gene sequencing. At the phylum level, the control group (0 mg kg −1 SMM) was dominated by Actinobacteria, Proteobacteria, and Firmicutes. In the treatment groups, Firmicutes, Proteobacteria, and Chloroflexi were the dominant phyla. Cluster analysis indicated that the two groups treated with SMM clustered together. Similarly, the bacterial families that dominated the control group differed from those dominating the treatment groups. The changes in intestinal microbial composition over time were similar between the two SMM treatment groups. In both groups, the abundances of some families, including the Bacillaceae, Streptococcaceae, and Pseudomonadaceae, increased first and then decreased. Overall, the addition of SMM to the feed changed the structure of the intestinal microbiota in Nile tilapia. This study improves our understanding of the impact of SMM on the intestinal microenvironment of Nile tilapia. Our results provide guidelines for the feasibility of SMM use in aquaculture production.