Pseudomonas glycinae sp. nov. isolated from the soybean rhizosphere

Strains MS586 T and MS82, which are aerobic, Gram‐negative, rod‐shaped, and polar‐flagellated bacteria, were isolated from the soybean rhizosphere in Mississippi. Taxonomic positions of MS586 T and MS82 were determined using a polyphasic approach. 16S rRNA gene sequence analyses of the two strains showed high pairwise sequence similarities (>98%) to some Pseudomonas species. Analysis of the concatenated 16S rRNA, rpoB , rpoD , and gyrB gene sequences indicated that the strains belonging to the Pseudomonas koreensis subgroup (SG) shared the highest similarity with Pseudomonas kribbensis strain 46‐2 T . Analyses of average nucleotide identity (ANI), genome‐to‐genome distance, delineated MS586 T and MS82 from other species within the genus Pseudomonas . The predominant quinone system of the strain was ubiquinone 9 (Q‐9), and the DNA G+C content was 60.48 mol%. The major fatty acids were C 16:0 , C 17:0 cyclo, and the summed features 3 and 8 consisting of C 16:1 ω7c/C 16:1 ω6c and C 18:1 ω7c/C 18:1 ω6c, respectively. The major polar lipids were phosphatidylglycerol, phosphatidylethanolamine, and diphosphatidylglycerol. Based on these data, it is proposed that strains MS586 T and MS82 represent a novel species within the genus Pseudomonas . The proposed name for the new species is Pseudomonas glycinae , and the type strain is MS586 T (accession NRRL B‐65441 = accession LMG 30275).