Open AccessIsolation of a novel Saccharophagus species (Myt‐1) capable of degrading a variety of seaweeds and polysaccharides
Author(s) -
Sakatoku A.,
Wakabayashi M.,
Tanaka Y.,
Tanaka D.,
Nakamura S.
Publication year - 2012
Publication title -
microbiologyopen
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.881
H-Index - 36
ISSN - 2045-8827
DOI - 10.1002/mbo3.10
Subject(s) - thallus , biology , 16s ribosomal rna , botany , homology (biology) , polysaccharide , strain (injury) , dna , algae , gene , biochemistry , anatomy
A bacterial strain, Myt‐1, was isolated in Toyama Bay in Toyama Prefecture, Japan. Myt‐1 was capable of reducing the thalli of various seaweed species to single cell detritus particles. A 16S rDNA homology search revealed that the closest relative of Myt‐1 was Saccharophagus degradans 2–40 (CP000282; 100% similarity), which was first isolated in Chesapeake Bay in Virginia, USA. The Myt‐1 strain was capable of degrading more than 10 polysaccharides, almost all of which were also degraded by S . degradans 2–40. Analyses of alginase gene DNA sequence homology, DNA–DNA homology, and zymogram analysis of obtained polysaccharidases suggested that Myt‐1 was a new species of Saccharophagus . Thus, Myt‐1 is only the second species in this genus, which has contained only one strain and species since 1988, and was tentatively designated Saccharophagus sp. Myt‐1. Myt‐1 has considerable potential for reducing the volume of seaweed wastes, and for producing functional materials from seaweed substrate.