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Die Oberflächenbindung von Virion‐Antikörper‐Komplexen: Eine kinetische Untersuchung
Author(s) -
Boltovets P. M.,
Boyko V. R.,
Snopok B. A.
Publication year - 2013
Publication title -
materialwissenschaft und werkstofftechnik
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.285
H-Index - 38
eISSN - 1521-4052
pISSN - 0933-5137
DOI - 10.1002/mawe.201300106
Subject(s) - surface plasmon resonance , nicotiana tabacum , chemistry , exponential function , kinetic energy , biophysics , surface (topology) , surface plasmon , virus , analytical chemistry (journal) , materials science , plasmon , nanotechnology , chromatography , virology , biology , nanoparticle , biochemistry , physics , optoelectronics , mathematics , mathematical analysis , geometry , quantum mechanics , gene
The presence of various components in complex biological samples which interact with inhomogeneous sensor surface, results in stretched exponential behaviour of the sensor response. This general concept was demonstrated by surface plasmon resonance (SPR) measurements of virus‐antibody complexes from crude plant homogenates on a gold surface, covered with protein A. The development of the viral infection in the plant Nicotiana tabacum was studied by SPR and ELISA (enzyme‐linked immunosorbent assay) as an indicative approach; a good agreement between both methods was shown. The correlation between virus concentration and the parameters of stretched exponential law was demonstrated. These results indicate that the parameters have hidden intrinsic properties of the underlying interfacial reactions and give a good basis for a more specific microscopic model.