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Comparative study of biological action of fullerenes C 60 and carbon nanotubes in thymus cells
Author(s) -
Prylutska S.V.,
Grynyuk I.I.,
Grebinyk S.M.,
Matyshevska O.P.,
Prylutskyy Y.I.,
Ritter U.,
Siegmund C.,
Scharff P.
Publication year - 2009
Publication title -
materialwissenschaft und werkstofftechnik
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.285
H-Index - 38
eISSN - 1521-4052
pISSN - 0933-5137
DOI - 10.1002/mawe.200900433
Subject(s) - carbon nanotube , chemistry , atpase , incubation , hydrolysis , extracellular , enzyme , atp hydrolysis , membrane , fullerene , bioorganic chemistry , enzyme assay , biophysics , biochemistry , nanotechnology , materials science , organic chemistry , biology
Abstract In a model experiment on T‐cells isolated from Wistar rat thymus the biological activity of nonmodified fullerenes (C 60 ) and multi‐walled carbon nanotubes (MWCNT) is studied. It is demonstrated that C 60 at a concentration of 14.4 μg/ml in the cell incubation medium inhibit the activity of plasma membrane Ca 2+ ‐stimulated ecto‐ATPase. Both, the initial rate of the extracellular ATP hydrolysis and the time of reaching the half‐maximum level of the enzyme activity are decreased, while MWCNT (12.5 and 25 μg/ml) have no effect on the kinetic parameters of the ecto‐ATPase reaction. It is shown that both C 60 and MWCNT demonstrate a protective effect against H 2 O 2 ‐induced injury of thymic T‐cells.