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Erkennung von sterbenden Zellen mit Hilfe von Lektine – Verbindung fluoreszierender und lumineszierender Nanopartikel
Author(s) -
Bilyy R.,
Tomyn A.,
Kit Y.,
Podhorodecki A.,
Misiewicz J.,
Nyk M.,
Strek W.,
Stoika R.
Publication year - 2009
Publication title -
materialwissenschaft und werkstofftechnik
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.285
H-Index - 38
eISSN - 1521-4052
pISSN - 0933-5137
DOI - 10.1002/mawe.200900432
Subject(s) - fluorescence , conjugated system , nanoparticle , nanotechnology , nanobiotechnology , photobleaching , nanomaterials , chemistry , luminescence , lectin , cancer cell , biophysics , materials science , biochemistry , cancer , polymer , biology , optoelectronics , physics , organic chemistry , quantum mechanics , genetics
Constantly increasing sector of industry of biomedical diagnostics requires photosensitive and stable nanomaterials which can be specifically bio‐labeled for targeting selected cells in the body. Here we describe two types of novel nanomaterials conjugated with specific proteins recognized by dying (apoptotic and necrotic) cells. They are luminescent GaN:Eu 3+ ‐doped nanoparticles with wide green emission and very narrow red emission and fluorescent green‐emitting fluorescein‐containing polystyrene nanoparticles. These nanoparticles were conjugated with carbohydrate recognizing proteins – the lectins – that are targeting specific cell surface glycoproteins which were recently shown to be novel markers of dying cells (Bilyy et al., 2003, Bilyy et al., 2007). Both luminescent and fluorescent nanoparticles possessed high brightness and low level of photobleaching and efficiently interacted with dying cells, thus discriminating them from the intact ones.