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Protective effects of C 60 fullerenes on staurosporine‐, cytosine arabinoside‐ and hydrogen peroxide‐induced cell death of normal but not of transformed T lymphocytes
Author(s) -
Palyvoda K.,
Samoylenko A.,
Drobot L.,
Matyshevska O.,
Scharff P.
Publication year - 2009
Publication title -
materialwissenschaft und werkstofftechnik
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.285
H-Index - 38
eISSN - 1521-4052
pISSN - 0933-5137
DOI - 10.1002/mawe.200800447
Subject(s) - jurkat cells , staurosporine , chemistry , apoptosis , microbiology and biotechnology , programmed cell death , t cell , biology , biochemistry , immunology , signal transduction , immune system , protein kinase c
C 60 fullerenes are carbon nanospheres known to exert a protective activity in several models of apoptotic cell death. Both normal rat thymocytes and human T‐cell lymphoma Jurkat cells were treated with staurosporine, cytosine arabinoside and hydrogen peroxide as agents that trigger cell death. Activation of caspase‐3 as apoptosis marker was demonstrated in cells incubated for 4 h in the presence of these agents at concentrations causing 50 % cell viability reduction. Addition of C 60 fullerenes (10 ‐5 M) protected normal T cells but not human T lymphoma cells from apoptosis induced either by staurosporine, cytosine arabinoside or hydrogen peroxide. These data demonstrate selectivity of fullerenes C 60 biological effects in cultures of normal and transformed T cells.