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Synthesis of PVP‐coated silver nanoparticles and their biological activity towards human mesenchymal stem cells
Author(s) -
Kittler S.,
Greulich C.,
Köller M.,
Epple M.
Publication year - 2009
Publication title -
materialwissenschaft und werkstofftechnik
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.285
H-Index - 38
eISSN - 1521-4052
pISSN - 0933-5137
DOI - 10.1002/mawe.200800437
Subject(s) - silver nanoparticle , silver nitrate , dynamic light scattering , polyvinylpyrrolidone , ethylene glycol , nanoparticle , reagent , zeta potential , nuclear chemistry , scanning electron microscope , materials science , chemistry , chemical engineering , nanotechnology , polymer chemistry , organic chemistry , engineering , composite material
Silver nanoparticles were prepared by the polyol process, i.e. by the reduction of silver nitrate with ethylene glycol in the presence of polyvinylpyrrolidone, PVP. Thereby, the silver nanoparticles were colloidally stabilized by the polymer. The synthesis of nanoparticles of different size and shape (cubes, rods and spheres) was possible by changing the reaction conditions such as reagent ratio and temperature. The silver nanoparticles were characterized by dynamic light scattering (DLS), zeta‐potential measurements, UV‐spectroscopy, and scanning electron microscopy (SEM). The biological activity of spherical PVP‐coated silver nanoparticles (about 100 nm diameter) was tested on human mesenchymal stem cells (hMSC) in comparison with equivalent amounts of silver ions (silver acetate). hMSC were treated with silver concentrations in the range of 50 ng mL ‐1 to 50 μg mL ‐1 for 7 days under cell culture conditions. Cytotoxic cell reactions occurred at ≥2.5 μg Ag mL ‐1 for nanoparticles and ≥1 μg Ag mL ‐1 for silver acetate, indicating a critical role of the silver ions for toxic reactions.