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Heat‐treated BMP‐2 depots release BMP‐2 in its bioactive form
Author(s) -
Winkler L.,
Müller R.,
Wiemann M.
Publication year - 2006
Publication title -
materialwissenschaft und werkstofftechnik
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.285
H-Index - 38
eISSN - 1521-4052
pISSN - 0933-5137
DOI - 10.1002/mawe.200600016
Subject(s) - bone morphogenetic protein 2 , alkaline phosphatase , chemistry , in vivo , cancellous bone , bone morphogenetic protein , titanium , biomedical engineering , bone morphogenetic protein 7 , in vitro , biophysics , microbiology and biotechnology , biochemistry , anatomy , biology , enzyme , organic chemistry , medicine , gene
Small depots of locally fixed bone morphogenetic protein‐2 (BMP‐2), which were dried onto biocompatible carriers and afterwards heat‐treated, induce a local differentiation of contacting osteoblastic cells. Here we investigate whether such depots may act via a release of bioactive BMP‐2. For in vivo experiments we employed a modified gap‐healing approach using dump bell‐shaped implants made from titanium plasma‐sprayed Ti‐6Al‐4V, which were coated with BMP‐2 at only one side. Four weeks after implantation into the cancellous bone of the lateral femur condylus of adult sheep we observed a polar formation of new trabecular bone close to the side where BMP‐2 had been immobilized. We also tested whether BMP‐2 depots stimulate remote cells under cell culture conditions and found that alkaline phosphatase (ALP) of MC3T3‐E1 cells was induced over a distance of several millimetres. Experiments show that BMP‐2 at least in part desorbs from air‐dried and heat‐treated BMP‐2 depots in its bioactive form. Local depots of BMP‐2 may therefore allow to improve bone formation next to metal implants at predefined sites.