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Biocompatibility of a nickel‐free austenitic steel assayed by osteoblastic MC3T3‐E1 cells
Author(s) -
Mölders M.,
Fischer A.,
Wiemann M.
Publication year - 2002
Publication title -
materialwissenschaft und werkstofftechnik
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.285
H-Index - 38
eISSN - 1521-4052
pISSN - 0933-5137
DOI - 10.1002/mawe.200290010
Subject(s) - biocompatibility , alkaline phosphatase , intracellular , in vitro , osteoblast , austenitic stainless steel , corrosion , chemistry , cell culture , ductility (earth science) , bone cell , metallurgy , materials science , biophysics , biomedical engineering , microbiology and biotechnology , biochemistry , biology , enzyme , medicine , creep , genetics
The in vitro biocompatibility of the nickel‐free austenitic steel (brand name P2000) characterized by extreme strength, high ductility and superior corrosion resistance was tested. As the material appears promising for hard tissue implant development we employed osteoblastic cells (MC3T3‐E1) as test specimens. Cells growing directly on this metal were undistinguishable from control cells on cell culture tested plastic material with respect to morphology and growth parameters. P2000 can, therefore, be classified as a biocompatible material. In line with that application of bone morphogentic protein 2 (BMP‐2) increased expression of alkaline phosphatase as it does under control conditions. However, the slope of the dose response curve was diminished, indicating partially impaired BMP‐2 signaling on P2000. Since P2000 contains nitrogen and may release alkalizing amounts of NH 3 we compared steady state intracellular pH (pHi) of cells on P2000 vs. controls, but were unable to find any differences. Further studies are needed to understand how P2000 can influence cellular functions of directly contacting cells.