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Computer simulation of DNA gel electrophoresis: influence of solid friction on linear and circular chains
Author(s) -
Widgren Stefan,
Elvingson Christer
Publication year - 1996
Publication title -
macromolecular theory and simulations
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.37
H-Index - 56
eISSN - 1521-3919
pISSN - 1022-1344
DOI - 10.1002/mats.1996.040050602
Subject(s) - circular dna , electrophoresis , molecule , brownian dynamics , linear polymer , materials science , electric field , chemical physics , gel electrophoresis , brownian motion , molecular dynamics , polyelectrolyte , linear molecular geometry , polymer , chemistry , composite material , physics , chromatography , computational chemistry , organic chemistry , biochemistry , quantum mechanics , genome , gene
The effect of solid friction between DNA molecules and gel fibres during gel electrophoresis has been studied using Brownian dynamics simulations. To investigate the effect of the topology of the polyelectrolyte chains, we have studied both linear and circular molecules. The results show that the migration properties of linear molecules seem to be almost uneffected by solid friction. There is, however, a significant effect on the mobility of circular molecules of the same size. Increasing the electric field strength increases the mobility of the linear molecules while for the circular chains, the mobility goes through a maximum when friction is included.