Premium
Exploration of Fluorescence Quenching Mechanism in Tryptophan Induced by Norfloxacin: Analytical Applications
Author(s) -
Mahanwar Sunanda T.,
Patil Shilpa R.,
Naik Vaibhav M.,
Waghmare Ravindra D.,
Anbhule Prashant V.,
Kolekar Govind B.
Publication year - 2019
Publication title -
macromolecular symposia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.257
H-Index - 76
eISSN - 1521-3900
pISSN - 1022-1360
DOI - 10.1002/masy.201800208
Subject(s) - quenching (fluorescence) , tryptophan , fluorescence , chemistry , norfloxacin , photochemistry , analytical chemistry (journal) , chromatography , optics , biochemistry , physics , amino acid , ciprofloxacin , antibiotics
Fluorescence quenching mechanism of tryptophan (Trp) by norfloxacin (NORF) was investigated by spectrofluorometric method at physiological pH. Stern−Volmer quenching constant is positively related with temperature shows the dynamic type of quenching. Life time of Trp decreases by the addition of NORF supports dynamic type of quenching. Fluorescence quenching method was successfully applied for direct determination of NORF from pharmaceutical sample.