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Dark Field Transmission Electron Microscopy Imaging for Biological and Soft Matter Systems
Author(s) -
Ayache Jeanne,
Péchoux Christine,
Jaillard Danielle,
Payré Bruno,
PignotPaintrand Isabelle,
Le Cam Eric
Publication year - 2016
Publication title -
macromolecular symposia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.257
H-Index - 76
eISSN - 1521-3900
pISSN - 1022-1360
DOI - 10.1002/masy.201500012
Subject(s) - dark field microscopy , transmission electron microscopy , materials science , nanotechnology , microscopy , macromolecule , biological specimen , optics , chemistry , physics , biochemistry
Summary In this study, we present a new application of the transmission electron microscopy dark field mode for cell imaging. We have applied this imaging mode to two types of cellular systems: human HeLa cells to analyze molecular membrane systems and HC11 mouse mammary cells containing lipid molecule droplets. We have also studied a third macromolecular system, copolymer nanoparticles for the characterization of core‐shell structures. We want to show the effective use of diffraction contrast, even on amorphous systems for increasing the image contrast and the signal/noise ratio. We discuss the TEM dark field advantages for the analysis of polymers and other macromolecular systems, including biological, systems compared to the bright field mode.