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Synthesis and Characterization of a Telechelic Peptide as a Precursor for Supramolecular Networks
Author(s) -
Federico Stefania,
Lendlein Andreas,
Neffe Axel T.
Publication year - 2011
Publication title -
macromolecular symposia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.257
H-Index - 76
eISSN - 1521-3900
pISSN - 1022-1360
DOI - 10.1002/masy.201100055
Subject(s) - peptide , supramolecular chemistry , mass spectrometry , two dimensional nuclear magnetic resonance spectroscopy , heteronuclear single quantum coherence spectroscopy , combinatorial chemistry , desorption , characterization (materials science) , matrix assisted laser desorption/ionization , chemistry , chromatography , materials science , stereochemistry , nanotechnology , organic chemistry , adsorption , molecule , biochemistry
A peptide showing propensity for the adoption of β‐sheet conformation was synthesized in order to develop a defined molecular building block as part of a toolbox for the development of polymer networks based on supramolecular interactions. The peptide was synthesized by microwave‐assisted solid phase peptide synthesis and the purification was performed by Reversed Phase‐High Performance Liquid Chromatography (RP‐HPLC), from which a purity higher than 95 mol‐% was obtained. The calculated mass was confirmed by Matrix Assisted Laser Desorption Ionization‐Time of Flight‐Mass Spectrometry (MALDI‐ToF‐MS). Further characterization of the peptide was performed by IR and 2D TOCSY, NOESY, and HSQC NMR spectroscopy, which confirmed the identity and the sequence of the peptide.