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Dynamics of the Alignment of Mammalian Cells on a Nano‐Structured Polymer Surface
Author(s) -
Peterbauer Thomas,
Yakunin Sergii,
Siegel Jakub,
Heitz Johannes
Publication year - 2010
Publication title -
macromolecular symposia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.257
H-Index - 76
eISSN - 1521-3900
pISSN - 1022-1360
DOI - 10.1002/masy.201051038
Subject(s) - chinese hamster ovary cell , microbiology and biotechnology , motility , cytoplasm , cell culture , in vitro , materials science , biophysics , chemistry , biology , biochemistry , genetics
In vitro cultured mammalian cells are able to sense micro‐ and nanotopographical features of their substratum, resulting in contact guidance of the cells along topographical features, altered motility, proliferation and differentiation. We here used embossed polyester films with nanogrooves to study the dynamics of cell spreading and alignment of chinese hamster ovary (CHO) cells and cells of a rat myogenic cell line. Our results suggest that the response of the cells studied is driven by initial cytoplasmic protrusions, which emerge along the nanogrooves and pull the cells along the direction of the nanogrooves.