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Mammalian Cell Culture on a Novel Chitosan‐Based Biomaterial Crosslinked with Gluteraldehyde
Author(s) -
FabelaSánchez O.,
ZarateTriviño D. G.,
ElizaldePeña E. A.,
GarcíaCarvajal Z.,
Sánchez I. C.,
ParraCid C.,
GómezGarcía R.,
Ibarra C.,
GarcíaGaitán B.,
Zavala R.,
GuevaraOlvera L.,
VillaseñorOrtega F.,
MuñozSanchez C. I.,
PerezPerez C.,
HerreraPerez S.,
Velasquillo C.,
LunaBarcenas G.
Publication year - 2009
Publication title -
macromolecular symposia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.257
H-Index - 76
eISSN - 1521-3900
pISSN - 1022-1360
DOI - 10.1002/masy.200950924
Subject(s) - biocompatibility , chitosan , glutaraldehyde , viability assay , biomaterial , materials science , glycidyl methacrylate , biomedical engineering , self healing hydrogels , dermal fibroblast , scaffold , fibroblast , in vitro , chemistry , biophysics , polymer chemistry , nanotechnology , polymer , biochemistry , chromatography , composite material , medicine , monomer , biology , metallurgy
A novel (Chitosan‐ g ‐Glycidyl Methacrylate)‐Xanthan, (CTS‐ g ‐GMA)‐X, hydrogel was successfully synthesized at Cinvestav Queretaro. In the present work, we attempt to study the in vitro biocompatibility of (CTS‐ g ‐GMA)‐X hydrogels as scaffolds for neural cells obtained from cerebral cortex of mice; skin cells from skin of mice; and chondrocytes from human cartilage. The results obtained in the live/dead assay show that the materials maintain the morphology and viability of the cells. However the high friability of the materials prevented an extended test time in this assay. Therefore, glutaraldehyde (GL) was added to the structure as crosslinking agent to enhance friability. All results point out that [(CTS‐g‐GMA)‐X]‐GL hydrogel is viable as a scaffold for chondrocytes and skin cells; and (CTS‐g‐GMA)‐X for neural cell due to their physicochemical properties and biocompatibility with the cells for both materials.