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Surface Modification of Polyurethanes with Covalent Immobilization of Heparin
Author(s) -
Aksoy Ayse E,
Hasirci Vasif,
Hasirci Nesrin
Publication year - 2008
Publication title -
macromolecular symposia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.257
H-Index - 76
eISSN - 1521-3900
pISSN - 1022-1360
DOI - 10.1002/masy.200850918
Subject(s) - heparin , polyurethane , covalent bond , adhesion , chemistry , polyol , surface modification , thrombus , polymer chemistry , materials science , whole blood , coating , biomedical engineering , organic chemistry , biochemistry , surgery , medicine
Thrombus formation and blood coagulation is a major problem associated with blood contacting products such as catheters, vascular grafts, arteries, artificial hearts and heart valves. An intense research is being conducted towards the synthesis of new hemocompatible materials and modifications of surfaces with biological molecules. In this study, polyurethane (PU) films were synthesized in medical purity from diisocyanate and polyol without using any other ingredients and their surfaces were modified by covalent immobilization of heparin. Two types of heparin, unfractionated (UFH) and low molecular weight heparin (LMWH), were immobilized to investigate their effect on cell adhesion. The surface properties of the modified PUs were examined with ESCA, ATR‐FTIR and AFM. ESCA results demonstrated sulfur peaks indicating the presence of heparin and AFM results showed the alteration of surface structure after coating with heparin. Cell adhesion studies were conducted with heparinized whole human blood. The surfaces of the UFH immobilized films resulted in lesser red blood cell adhesion in comparison to LMWH demonstrating strong anti‐thrombogenic activity of the latter.