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Expression Profile of pha Gene Cluster of Pseudomonas putida KT2442
Author(s) -
de Eugenio Laura I.,
Escapa Isabel F.,
Galán Beatriz,
García José L.,
Prieto María A.
Publication year - 2008
Publication title -
macromolecular symposia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.257
H-Index - 76
eISSN - 1521-3900
pISSN - 1022-1360
DOI - 10.1002/masy.200850902
Subject(s) - pseudomonas putida , polyhydroxyalkanoates , bacteria , pseudomonas , gene cluster , monomer , gene , biochemistry , pseudomonadales , homology (biology) , pseudomonadaceae , polyester , chemistry , polymer , biology , microbiology and biotechnology , organic chemistry , genetics
Polyhydroxyalkanoates (PHAs) are biodegradable polymers that many bacteria accumulate as carbon and energy storage when growth conditions are unbalanced. Pseudomonas strains belonging to the rRNA homology group I such as P. putida can accumulate medium‐chain‐length‐PHA from monomers in the C8 to C10 range. Regulation of PHA synthesis and degradation in P. putida KT2442 has been studied using different molecular approaches. In this study six promoter regions located upstream of each pha gene were identified. The expression of the pha cluster have been analysed in the presence of octanoic acid versus glucose in the culture medium. Results demonstrated that the system is activated in the presence of octanoic acid as PHA precursor.