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Preparation of Porous Protein‐Based Hydogel for Highly Sensitive Protein Chips
Author(s) -
Tanaka Hiroyuki,
Shiroya Toshifumi,
Hanasaki Minako,
Isojima Tatsushi,
Takeuchi Hisao,
Kawaguchi Haruma
Publication year - 2008
Publication title -
macromolecular symposia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.257
H-Index - 76
eISSN - 1521-3900
pISSN - 1022-1360
DOI - 10.1002/masy.200850615
Subject(s) - polymer , substrate (aquarium) , protein aggregation , materials science , colloidal gold , chemistry , nanotechnology , nanoparticle , biochemistry , biology , organic chemistry , ecology
Summary: Protein chips are important tools for high‐throughput analysis of biological events. We have developed a novel method to prepare a protein‐based hydrogel, that is, a “Three‐Dimensional Nano‐structured Protein Hydrogel” (3‐D NPH), which is composed of protein and polymer nano‐particles. The 3‐D NPH could be easily prepared by dispensing a protein and polymer mixture on a substrate. Surprisingly, gold particles conjugated with protein A diffused into the 3‐D NPH which was made of mouse IgG through the pores. We have shown that the protein chips made with our 3‐D NPH method has tremendously improved sensitivity in detecting protein‐protein interactions compared with that of direct protein immobilization methods.