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A new Phosphorylated Polysaccharide for Biomedical Applications: Generation of 3D Scaffolds with Osteogenic Activity and Coating of Titanium Oxide Surfaces
Author(s) -
Pasqui Daniela,
Danieli Elisa,
Leone Gemma,
Torricelli Paola,
Barbucci Rolando
Publication year - 2008
Publication title -
macromolecular symposia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.257
H-Index - 76
eISSN - 1521-3900
pISSN - 1022-1360
DOI - 10.1002/masy.200850605
Subject(s) - osseointegration , biomaterial , chemical engineering , polysaccharide , self healing hydrogels , surface modification , x ray photoelectron spectroscopy , coating , chemistry , simulated body fluid , osteoblast , biopolymer , phosphate , titanium , materials science , titanium oxide , nuclear chemistry , polymer chemistry , apatite , organic chemistry , biochemistry , in vitro , polymer , mineralogy , medicine , surgery , implant , engineering
Summary: A new phosphorylated derivative of carboxymethylcellulose and amidic carboxymethylcellulose containing one phosphate group for each disaccharide unit was synthesized using sodium trimetaphospahte (STMP) as the phosphating agent. The new polysaccharide was characterized by infrared spectroscopy (FT‐IR) and the amount of phosphate groups was determined by elemental analysis. These modified polysaccharides were used both to prepare 3D scaffolds and functionalize titanium oxide surfaces with the aim to improve the osseointegration with the host tissue. The presence of phosphate groups modify the physical‐chemical properties of the hydrogels with respect to the native ones. The evaluation of the bioactivity of the phosphorylated carboxymethylcellulose hydrogels towards osteoblast‐like cells showed a significant increase in the osteocalcin production. The modified surfaces were chemically characterized by means of X‐ray photoelectron spectroscopy (XPS) and FT‐IR, whereas the surface topography was analysed by Atomic Force Measurements (AFM) measurements before and after the polysaccharide coating. In vitro biological tests using osteoblast‐like cells demonstrated that phosphorylated carboxymethylcellulose functionalized TiO 2 surfaces promoted better cell adhesion and significantly enhanced their proliferation. These findings suggest that the phosphate polysaccharide both as a 3D scaffold and as a surface coating promotes osteoblast growth potentially improving the biomaterial osseointegration rate.