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Chemo‐enzymatic Synthesis and RAFT Polymerization of 6‐ O ‐Methacryloyl Mannose: A Suitable Glycopolymer for Binding to the Tetrameric Lectin Concanavalin A?
Author(s) -
Granville Anthony M.,
Quémener Damien,
Davis Thomas P.,
BarnerKowollik Christopher,
Stenzel Martina H.
Publication year - 2007
Publication title -
macromolecular symposia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.257
H-Index - 76
eISSN - 1521-3900
pISSN - 1022-1360
DOI - 10.1002/masy.200750909
Subject(s) - glycopolymer , concanavalin a , raft , reversible addition−fragmentation chain transfer polymerization , monomer , chain transfer , mannose , polymerization , chemistry , polymer chemistry , radical polymerization , organic chemistry , biochemistry , polymer , in vitro
Summary: The chemo‐enzymatic synthesis of 6‐ O ‐methacryloyl mannose (MaM) glycomonomer was successfully performed for the first time. Subsequent aqueous RAFT polymerization of the monomer yielded well‐defined, linear poly(6‐ O ‐methacryloyl mannose) (PMaM) glycopolymers without the need for protecting and deprotecting group chemistry. As well as investigating the RAFT polymerization kinetics of this monomer using various initial monomer to chain transfer agent concentration ratios, the protein binding ability of the generated glycopolymer was tested using concanavalin A, a known mannose‐residue binding lectin.