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Synthesis, Characterisation of Novel Polyaniline Nanomaterials and Application in Amperometric Biosensors
Author(s) -
Michira I.,
Akinyeye R.,
Somerset V.,
Klink M. J.,
Sekota M.,
AlAhmed A.,
Baker P. G. L.,
Iwuoha E.
Publication year - 2007
Publication title -
macromolecular symposia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.257
H-Index - 76
eISSN - 1521-3900
pISSN - 1022-1360
DOI - 10.1002/masy.200750907
Subject(s) - biosensor , polyaniline , detection limit , amperometry , nuclear chemistry , horseradish peroxidase , sulfonic acid , chemistry , hydrogen peroxide , dodecylbenzene , materials science , polymerization , chromatography , electrode , organic chemistry , nanotechnology , electrochemistry , polymer , sulfonate , sodium , enzyme
Summary: Anthracene sulfonic acid doped polyaniline nanomaterials were prepared through the chemical oxidative polymerisation process. Ammonium peroxydisulfate (APS) was employed as oxidant. Scanning electron microscopy (SEM) results show the resultant polyaniline (PANi) materials exhibited nanofibrillar morphology with diameter sizes less than 300 nm. Using the nanofibrillar PANI, amperometric biosensors for H 2 O 2 and erythromycin were constructed through the drop‐coating technique. Anthracene sulfonic acid (ASA) doped PANi and the test enzymes horseradish peroxidase, (HRP), or cytochrome P 450 3A4, (CYP 450 3A4) were mixed in phosphate buffer solution before drop coating onto the electrode. The resultant biosensors displayed typical Michaelis‐Menten behaviour. The apparent Michaelis‐Menten constant obtained was 0.18 ± 0.01 mM and 0.80 ± 0.02 µM L −1 for the peroxide and erythromycin biosensor respectively. The sensitivity for the peroxide sensor was 3.3 × 10 −3 A · cm −2 · mM −1 , and the detection limit was found to be 1.2 × 10 −2 mM respectively. Similarly, the sensitivity for the erythromycin sensor was in the same order at 1.57 × 10 −3 A · cm −2 · mM −1 and detection limit was found to be 7.58 × 10 −2 µM.