Premium
Preparation of Cationic Comb‐Type Copolymers Having High Density of PEG Graft Chains for Gene Carriers
Author(s) -
Choi Sung Won,
Yamayoshi Asako,
Hirai Miwa,
Yamano Takeshi,
Takagi Motoki,
Sato Ayumi,
Kano Arihiro,
Shimamoto Akira,
Maruyama Atsushi
Publication year - 2007
Publication title -
macromolecular symposia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.257
H-Index - 76
eISSN - 1521-3900
pISSN - 1022-1360
DOI - 10.1002/masy.200750351
Subject(s) - cationic polymerization , copolymer , polymer chemistry , peg ratio , reductive amination , polyethylene glycol , aqueous solution , polymer , side chain , chemistry , acetonitrile , materials science , organic chemistry , finance , economics , catalysis
Abstract A series of poly(L‐lysine)‐ graft ‐polyethylene glycol (PLL‐ g ‐PEG) was prepared by a reductive amination reaction of PLL with aldehyde modified PEG using NaBH 3 CN as a reductant. The high coupling efficacy observed in the 80% acetonitrile aqueous solution led to the grafting polymers with abundant PEG chains (more than 90 wt % and 30 mol %). By the method, we can retain high cationic charge density of the polymer backbone, because the resulting secondary amino groups are still protonated. By controlling the primary structure of the copolymer, we can also regulate the ionic interaction with nucleic acids and other biological components for gene delivery purpose.