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Synthesis and Evaluation of PEG Derivatives for Crosslinking Cells
Author(s) -
Ito Michiko,
Tateishi Tetsuya,
Taguchi Tetsushi
Publication year - 2007
Publication title -
macromolecular symposia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.257
H-Index - 76
eISSN - 1521-3900
pISSN - 1022-1360
DOI - 10.1002/masy.200750326
Subject(s) - spheroid , ethylene glycol , peg ratio , polymer chemistry , membrane , fourier transform infrared spectroscopy , gel permeation chromatography , permeation , chemistry , materials science , chemical engineering , organic chemistry , polymer , biochemistry , finance , economics , engineering , in vitro
The objective of this study is synthesis, characterization, and evaluation of a novel crosslinker to form spheroids. Our approach was based on the crosslinking of cell membrane using a polymeric crosslinker via hydrophobic interaction. A crosslinker poly(ethylene glycol) derivative with oleyl groups as hydrophobic group at both ends was synthesized, and then characterized with gel permeation chromatography and Fourier‐transform infrared spectroscopy. Furthermore, cell culture experiments were performed to confirm spheroid formation. Spheroids were successfully obtained when the crosslinker was added to cell suspension. This polymeric crosslinker was useful in the formation of cell spheroid.